Journal: Theranostics

Article Title: Anti-angiogenic Nanotherapy Inhibits Airway Remodeling and Hyper-responsiveness of Dust Mite Triggered Asthma in the Brown Norway Rat

doi: 10.7150/thno.16627

Figure Lengend Snippet: A. Scheme 1 Synthesis of fumagillin (fumagillol) PAz-PC prodrug. B. Scheme 2 Synthesis of docetaxel prodrug. C. Structure α v β 3 -peptidomimetic-PEG 2000 -PE. D. Example of dynamic light scattering micelle particle size distribution (α v β 3 -Dxtl-PD). E. Averaged pharmacokinetic profile of α v β 3 -micelles containing a Gd 3+ -lipid marker in the surfactant commixture. The two compartment model Gd 3+ concentration (C) = A e -αt + B e -βt revealed an alpha distribution half-life of 6.9 min and a beta elimination half-life of 154.6 min.

Article Snippet: The α v β 3 -peptidomimetic homing ligand utilized was provided as a gift (Kereos, Inc, St. Louis). ( Figure C ) α v β 3 -mixed micelles used for drug delivery had nominal particle sizes of 16 ± 4 nm and polydispersities between 0.1 and 0.2 based on dynamic light scattering (DLS) and an electrophoretic potential (ζ) of -20 ± 6 mV, across batches.

Techniques: Marker, Concentration Assay

Journal: Scientific Reports

Article Title: The oncolytic adenovirus VCN-01 promotes anti-tumor effect in primitive neuroectodermal tumor models

doi: 10.1038/s41598-019-51014-1

Figure Lengend Snippet: Characterization of VCN-01 in PNET cell lines in vitro . ( A ) Expression of adenoviral receptors CAR, α v β 3 integrin and α v β 5 integrin in CNS-PNET cell lines PFSK-1 (left) and SK-PN-DW (right). Graph shows the percentage of stained cells for each receptor (Mean ± SD; n = 3). ( B ) PFSK-1 and SK-PN-DW (200,000 cells) infected with the GFP expressing vector AdTLRGDK at MOIs of 0, 0.1, 1, 10 or 100 PFU/cell. Graph indicates the percentage of GFP positive cells expression measured by flow cytometry at 24 h (white bars) or 48 h (black bars) after the infection (Mean ± SD; n = 3). ( C ) Detection of the viral proteins E1A and fiber in whole-cell lysates 48 h after being from PFSK-1 and SK-PN-DW from VCN-01 infected PFSK-1 and SK-PN-DW cultures. Grb2 was used as loading control protein. Blots from different parts of the same gel have been grouped to improve clarity of the image. ( D ) Viral titers in PFSK-1 and SK-PN-DW (50,000 cells/well) cultures 72 h after being infected with VCN-01 at MOIs 1 and 10. Bars represent total PFUs contained in the lysates (Mean ± SD; n = 3).

Article Snippet: PNET cell lines PFSK-1 (ATCC, Manassas, VA, USA; CRL-2060 TM ) and SK-PN-DW (ATCC, CRL-2139 TM ) were stained with unlabeled monoclonal antibodies recognizing the adenoviral receptors CAR (Merck Millipore, Temecula, CA, USA; 05–644) α v β 3 integrin (Merck Millipore; CBL544) and α v β 5 integrin (R&D Systems Inc., Minneapolis, MN, USA; MAB2528), and donkey anti-mouse IgG1-AlexaFluor488 (Thermo Fisher Scientific, Waltham, MA, USA; A-21202) as secondary antibody.

Techniques: In Vitro, Expressing, Staining, Infection, Plasmid Preparation, Flow Cytometry, Control

Journal: Breast Cancer Research

Article Title: β 3 Integrin and Src facilitate transforming growth factor-β mediated induction of epithelial-mesenchymal transition in mammary epithelial cells

doi: 10.1186/bcr1524

Figure Lengend Snippet: TGF-β 1 mediated EMT increases cell surface α v β 3 integrin expression in NMuMG cells. NMuMG cells were incubated in the absence or presence of TGF-β 1 (5 ng/ml) for 0–36 hours and assayed for EMT. (a) Alterations in actin cytoskeletal architecture were visualized by direct rhodamine-phalloidin immunofluorescence and α v β 3 integrin by indirect immunofluorescence using anti-α v β 3 integrin (LM609) antibodies. Shown are representative images from a single experiment that was repeated twice with identical results. (b) Detergent-solubilized whole cell extracts (50 μg/lane) were prepared to analyze expression of the α v and β 3 integrin subunits by immunoblotting. Differences in protein loading were monitored by reprobing striped blots with anti-ERK1/2 antibodies because, unlike the increase in β-actin expression observed (data not shown) ERK1/2 expression was unaltered in NMuMG cells in response to TGF-β 1 treatment. (c) β 3 Integrin deficient NMuMG cells were generated by siRNA transfection. The effects of β 3 integrin deficiency on TGF-β mediated EMT was visualized by direct FITC-phalloidin immunofluorescence. Shown are representative images from a single experiment that was repeated twice with identical results. Detergent-solubilized whole cell extracts (50 μg/lane) were prepared to analyze expression of the α v , β 3 , and β 1 integrin subunits by immunoblotting. Differences in protein loading were monitored by reprobing striped blots with anti-β-actin antibodies. EMT, epithelial-mesenchymal transitions; ERK, extracellular signal-regulated kinase; siRNA, small interfering RNA; TGF, transforming growth factor.

Article Snippet: For α v β 3 integrin staining, the cells were blocked in goat β-globulin (200 μg/ml; Jackson Immunoresearch, West Grove, PA, USA) before sequential incubations with anti-α v β 3 LM609 antibody (1:250 dilution; BD Biosciences, San Jose, CA USA), followed by biotinylated goat anti-mouse antibody (5 μg/ml; Jackson Immunoresearch) and finally by Alexa-streptavidin (1.2 μg/ml; Invitrogen).

Techniques: Expressing, Incubation, Immunofluorescence, Western Blot, Generated, Transfection, Small Interfering RNA

Journal: Molecular cancer therapeutics

Article Title: Targeted radionuclide therapy in patient-derived xenografts using 177 Lu-EB-RGD

doi: 10.1158/1535-7163.MCT-19-1098

Figure Lengend Snippet: Histopathology and positron-emission tomography (PET) imaging of patient-derived xenografts (PDX) and parental tumours. (A) Haematoxylin and eosin (H&E) staining in human NSCLCs and corresponding PDXs; (B) Immunohistochemistry staining of integrin αvβ3 expression and CD31 in human NSCLCs and corresponding PDXs (tumour vasculatures were indicated by arrows); (C) The expression of integrin αvβ3 was detected by Western blot analysis; (D) 18F-FDG PET imaging in two patients with NSCLC and corresponding PDXs.

Article Snippet: The primary antibodies of this experiment were anti-mouse CD31 mAb (ab9498; Abcam, Cambridge, United Kingdom), Ki-67-specific mAb (ab15580; Abcam, Cambridge, United Kingdom), human integrin α v β 3 (ab7166; Abcam, Cambridge, United Kingdom), and murine integrin α v β 3 (CD61, Novus Biologicals, SJ19–09).

Techniques: Histopathology, Positron Emission Tomography, Imaging, Derivative Assay, Staining, Immunohistochemistry, Expressing, Western Blot

Journal: Molecular cancer therapeutics

Article Title: Targeted radionuclide therapy in patient-derived xenografts using 177 Lu-EB-RGD

doi: 10.1158/1535-7163.MCT-19-1098

Figure Lengend Snippet: Therapy protocol and outcomes in PDXαvβ3- mice (A) Design of therapy protocol in the NSCLC-PDXs with low integrin expression (PDXαvβ3-). (B) Change in body weight after treatment. (C) Change in tumour volume after treatment.

Article Snippet: The primary antibodies of this experiment were anti-mouse CD31 mAb (ab9498; Abcam, Cambridge, United Kingdom), Ki-67-specific mAb (ab15580; Abcam, Cambridge, United Kingdom), human integrin α v β 3 (ab7166; Abcam, Cambridge, United Kingdom), and murine integrin α v β 3 (CD61, Novus Biologicals, SJ19–09).

Techniques: Expressing